Supplemental Material 2


Supplemental Material 2: Quantitative phosphoproteomic analysis of proteins not known to function in TCR signaling in wild type cells.  Label free heatmap of a portion of the data collected, representing proteins that were observed in our study of human Jurkat P116.c139 (Zap-70 reconstituted) cells not known to participate in the canonical T cell signaling pathway.  Temporal quantitative changes in phosphorylation state are represented as heatmaps.  Heatmaps are calculated from the average of 5 replicate experiments.  Yellow represents levels of phosphorylation above the average, while blue represents levels of phosphorylation below the average.  Black represents average abundance for a certain peptide across all time points.  Blanks in the heatmap indicate that a clearly defined SIC peak was not observed for that phosphopeptide in that time point.  Note that the * next to the phosphorylation site signifies that this site previously has been described in literature.

Back to Supplemental PageSupplemental_Material_T_cell.html